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Escherichia coli lux biosensors were constructed by inserting H-NS-dependent promoters into a vector, thereby placing each fragment upstream of the promoterless Photorhabdus luminescens luxCDABE operon. It was demonstrated that the DNA-mimic proteins ArdA, Arn and Ocr activate the transcription of H-NS-dependent promoters of the lux operon of marine luminescent bacteria mesophilic Aliivibrio fischeri and psychrophilic Aliivibrio logei , and the dps gene from E. It was also demonstrated that the ArdA antirestriction protein , the genes of which are located on transmissive plasmids ColIb-P9, R64, PK, decreases levels of H-NS silencing of the PluxC promoter during conjugation in the recipient bacteria.
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